DOI: 10.1111/gbi.12082
Scopus记录号: 2-s2.0-84898924076
论文题名: Prokaryote populations of extant microbialites along a depth gradient in Pavilion Lake, British Columbia, Canada
作者: Russell J.A. ; Brady A.L. ; Cardman Z. ; Slater G.F. ; Lim D.S.S. ; Biddle J.F.
刊名: Geobiology
ISSN: 1472-4677
EISSN: 1472-4669
出版年: 2014
卷: 12, 期: 3 起始页码: 250
结束页码: 264
语种: 英语
Scopus关键词: Bacteria (microorganisms)
; Cyanobacteria
; Prokaryota
; carbonic acid derivative
; RNA 16S
; archaeon
; Bacteria
; bacterial phenomena and functions
; biota
; Canada
; classification
; DNA barcoding
; genetics
; isolation and purification
; lake
; metabolism
; microbiology
; molecular genetics
; phylogeny
; physiology
; polymerase chain reaction
; sediment
; sequence analysis
; Archaea
; Bacteria
; Bacterial Physiological Phenomena
; Biota
; British Columbia
; Carbonates
; DNA Barcoding, Taxonomic
; Geologic Sediments
; Lakes
; Molecular Sequence Data
; Phylogeny
; Polymerase Chain Reaction
; RNA, Ribosomal, 16S
; Sequence Analysis, RNA
Scopus学科分类: Earth and Planetary Sciences: General Earth and Planetary Sciences
; Environmental Science: General Environmental Science
; Agricultural and Biological Sciences: Ecology, Evolution, Behavior and Systematic
英文摘要: Pavilion Lake in British Columbia, Canada, is home to modern-day microbialites that are actively growing at multiple depths within the lake. While microbialite morphology changes with depth and previous isotopic investigations suggested a biological role in the formation of these carbonate structures, little is known about their microbial communities. Microbialite samples acquired through the Pavilion Lake Research Project (PLRP) were first investigated for phototrophic populations using Cyanobacteria-specific primers and 16S rRNA gene cloning. These data were expounded on by high-throughput tagged sequencing analyses of the general bacteria population. These molecular analyses show that the microbial communities of Pavilion Lake microbialites are diverse compared to non-lithifying microbial mats also found in the lake. Phototrophs and heterotrophs were detected, including species from the recently described Chloroacidobacteria genus, a photoheterotroph that has not been previously observed in microbialite systems. Phototrophs were shown as the most influential contributors to community differences above and below 25 meters, and corresponding shifts in heterotrophic populations were observed at this interface as well. The isotopic composition of carbonate also mirrored this shift in community states. Comparisons to previous studies indicated this population shift may be a consequence of changes in lake chemistry at this depth. Microbial community composition did not correlate with changing microbialite morphology with depth, suggesting something other than community changes may be a key to observed variations in microbialite structure. © 2014 John Wiley & Sons Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85198
Appears in Collections: 影响、适应和脆弱性
There are no files associated with this item.
作者单位: School of Marine Science and Policy, University of Delaware, Lewes, DE, United States; Department of Biological Sciences, University of Calgary, Calgary, AB, Canada; University of North Carolina at Chapel Hill, Chapel Hill, NC, United States; School of Geography and Earth Sciences, McMaster University, Hamilton, ON, Canada; NASA Ames Research Center, Moffet Field, CA, United States; SETI Institute, Mountain View, CA, United States; School of Geography and Earth Sciences, McMaster University, Hamilton, ON, Canada
Recommended Citation:
Russell J.A.,Brady A.L.,Cardman Z.,et al. Prokaryote populations of extant microbialites along a depth gradient in Pavilion Lake, British Columbia, Canada[J]. Geobiology,2014-01-01,12(3)