DOI: 10.1016/j.marpolbul.2014.06.049
Scopus记录号: 2-s2.0-84922590545
论文题名: A selection of reference genes and early-warning mRNA biomarkers for environmental monitoring using Mytilus spp. as sentinel species
作者: Lacroix C. ; Coquillé V. ; Guyomarch J. ; Auffret M. ; Moraga D.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 86, 期: 2018-01-02 起始页码: 304
结束页码: 313
语种: 英语
英文关键词: Biomonitoring
; MRNA
; Mytilus spp.
; Pollution
; QPCR
; Reference genes
Scopus关键词: Environmental engineering
; Genes
; Molluscs
; Multivariant analysis
; Pollution
; Polymerase chain reaction
; Biomonitoring
; MRNA
; Mytilus
; QPCR
; Reference genes
; Biomarkers
; alpha tubulin
; biological marker
; caspase 2
; caspase 3
; caspase 8
; catalase
; complementary DNA
; copper zinc superoxide dismutase
; cytochrome P450
; cytochrome P450 3A
; glutathione peroxidase
; glutathione transferase
; glyceraldehyde 3 phosphate dehydrogenase
; messenger RNA
; metallothionein
; polycyclic aromatic hydrocarbon
; reactive oxygen metabolite
; ribosome protein
; RNA 18S
; biological marker
; messenger RNA
; water pollutant
; abiotic stress
; antioxidant activity
; Article
; biological monitoring
; biotransformation
; digestive system
; ecotoxicology
; environmental health
; environmental monitoring
; gene expression
; gill
; Mytilus
; Mytilus edulis
; Mytilus galloprovincialis
; nonhuman
; ovary cycle
; pollutant
; pollution
; polymerase chain reaction
; protein synthesis
; quantitative analysis
; real time polymerase chain reaction
; RNA extraction
; water quality
; analysis
; animal
; drug effects
; environmental monitoring
; evaluation study
; gastrointestinal tract
; gene
; gene expression regulation
; genetics
; metabolism
; multivariate analysis
; Mytilus
; procedures
; toxicity
; water pollutant
; Mytilus
; Animals
; Biomarkers
; Environmental Monitoring
; Gastrointestinal Tract
; Gene Expression Regulation
; Genes
; Gills
; Multivariate Analysis
; Mytilus
; RNA, Messenger
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: mRNA biomarkers are promising tools for environmental health assessment and reference genes are needed to perform relevant qPCR analyses in tissue samples of sentinel species. In the present study, potential reference genes and mRNA biomarkers were tested in the gills and digestive glands of native and caged mussels (Mytilus spp.) exposed to harbor pollution. Results highlighted the difficulty to find stable reference genes in wild, non-model species and suggested the use of normalization indices instead of single genes as they exhibit a higher stability. Several target genes were found differentially expressed between mussel groups, especially in gills where cyp32, π-gst and CuZn. -sod mRNA levels could be biomarker candidates. Multivariate analyses confirmed the ability of mRNA levels to highlight site-effects and suggested the use of several combined markers instead of individual ones. These findings support the use of qPCR technology and mRNA levels as early-warning biomarkers in marine monitoring programs. © 2014 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85731
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: LEMAR, UMR 6539, Institut Universitaire Européen de la Mer, Plouzané, France; CEDRE, 715, Rue Alain Colas, CS 41836, Brest Cedex 2, France
Recommended Citation:
Lacroix C.,Coquillé V.,Guyomarch J.,et al. A selection of reference genes and early-warning mRNA biomarkers for environmental monitoring using Mytilus spp. as sentinel species[J]. Marine Pollution Bulletin,2014-01-01,86(2018-01-02)