DOI: 10.1016/j.marpolbul.2016.09.044
Scopus记录号: 2-s2.0-85003904050
论文题名: Horizontal gene transfer versus biostimulation: A strategy for bioremediation in Goa
作者: Pasumarthi R. ; Mutnuri S.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2016
卷: 113, 期: 2018-01-02 起始页码: 271
结束页码: 276
语种: 英语
英文关键词: Bioaugmentation
; Biostimulation
; Horizontal gene transfer
; Hydrocarbon degradation
Scopus关键词: Biodegradation
; Bioremediation
; Biotechnology
; Diesel fuels
; Electrophoresis
; Gas chromatography
; Gene transfer
; Hydrocarbons
; Pollution
; Site selection
; Bio-augmentation
; Biostimulation
; Contaminated sites
; Gradient gel electrophoresis
; Horizontal gene transfer
; Hydrocarbon degradation
; Nonaqueous phase liquids
; Pseudomonas aeruginosa
; Genes
; diesel fuel
; sea water
; gasoline
; hydrocarbon
; water pollutant
; biodegradation
; bioremediation
; catabolism
; electrokinesis
; gas chromatography
; gene flow
; hydrocarbon
; marine pollution
; nonaqueous phase liquid
; oil pollution
; reliability analysis
; alkB gene
; aqueous solution
; Article
; bacterial gene
; bioaugmentation
; bioremediation
; biostimulated bioaugmentation
; biostimulation
; controlled study
; denaturing gradient gel electrophoresis
; experimental study
; gas chromatography
; horizontal gene transfer
; India
; intermethod comparison
; NDO gene
; nonhuman
; Pseudomonas aeruginosa
; reliability
; seashore
; stimulation
; waste water management
; water contamination
; oil spill
; water pollutant
; Goa
; India
; Pseudomonas aeruginosa
; Biodegradation, Environmental
; Gasoline
; Gene Transfer, Horizontal
; Hydrocarbons
; India
; Petroleum Pollution
; Pseudomonas aeruginosa
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Bioaugmentation, Biostimulation and Horizontal gene transfer (HGT) of catabolic genes have been proven for their role in bioremediation of hydrocarbons. It also has been proved that selection of either biostimulation or bioremediation varies for every contaminated site. The reliability of HGT compared to biostimulation and bioremediation was not tested. The present study focuses on reliability of biostimulatiion, bioaugmentation and HGT during biodegradation of Diesel oil and Non aqueous phase liquids (NAPL). Pseudomonas aeruginosa (AEBBITS1) having alkB and NDO genes was used for bioaugmentation and the experiment was conducted using seawater as medium. Based on Gas chromatography results diesel was found to be degraded to 100% in both presence and absence of AEBBITS1. Denturing gradient gel electrophoresis result showed same pattern in presence and absence of AEBBITS1 indicating no HGT. NAPL degradation was found to be more by Biostimulated Bioaugmentation compared to biostimulation and bioaugmentation alone. This proves that biostimulated bioaugmentation is better strategy for oil contamination (tarabll) in Velsao beach, Goa. © 2016 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86576
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, BITS-Pilani, K K Birla Goa Campus, India
Recommended Citation:
Pasumarthi R.,Mutnuri S.. Horizontal gene transfer versus biostimulation: A strategy for bioremediation in Goa[J]. Marine Pollution Bulletin,2016-01-01,113(2018-01-02)