DOI: 10.1016/j.marpolbul.2016.02.054
Scopus记录号: 2-s2.0-84977834612
论文题名: Direct evidences on bacterial growth pattern regulating pyrene degradation pathway and genotypic dioxygenase expression
作者: Chen B. ; Huang J. ; Yuan K. ; Lin L. ; Wang X. ; Yang L. ; Luan T.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2016
卷: 105, 期: 1 起始页码: 73
结束页码: 80
语种: 英语
英文关键词: Bacterial growth
; Biodegradation
; Dioxygenase
; Polycyclic aromatic hydrocarbons
; Pyrene
Scopus关键词: Anthracene
; Aromatic hydrocarbons
; Bacteria
; Biodegradation
; Carbon
; Degradation
; Efficiency
; Nutrients
; Polycyclic aromatic hydrocarbons
; Transcription
; Bacterial growth
; Biological conversion
; Degradation efficiency
; Degradation pathways
; Degradation rate
; Dioxygenases
; Metabolic pathways
; Transcription level
; Pyrene
; 4,5 pyrenediol
; dioxygenase
; fluoranthene
; phenanthrene
; pyrene
; pyrene derivative
; unclassified drug
; dioxygenase
; fluorene derivative
; phenanthrene derivative
; polycyclic aromatic hydrocarbon
; water pollutant
; bacterium
; biodegradation
; genotype
; growth response
; PAH
; pyrene
; Article
; bacterial cell
; bacterial gene
; bacterial growth
; bacterial strain
; biodegradation
; contamination
; controlled study
; correlational study
; culture medium
; gene identification
; genetic transcription
; genotype
; molecular biology
; Mycobacterium
; nidA gene
; nidA3 gene
; nonhuman
; nutrient broth
; pdoA2 gene
; protein expression
; bioremediation
; genetics
; metabolism
; water pollutant
; Bacteria (microorganisms)
; Mycobacterium sp.
; Biodegradation, Environmental
; Dioxygenases
; Fluorenes
; Metabolic Networks and Pathways
; Mycobacterium
; Phenanthrenes
; Polycyclic Aromatic Hydrocarbons
; Pyrenes
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Pyrene degradation by Mycobacterium sp. strain A1-PYR was investigated in the presence of nutrient broth, phenanthrene and fluoranthene, respectively. Fast bacterial growth in the nutrient broth considerably enhanced pyrene degradation rate, whereas degradation efficiency per cell was substantially decreased. The addition of nutrient broth could not alter the transcription levels of all dioxygenase genotypes. In the PAH-only substrates, bacterial growth completely relied on biological conversion of PAHs into the effective carbon sources, which led to a higher degradation efficiency of pyrene per cell than the case of nutrient broth. Significant correlations were only observed between nidA-related dioxygenase expression and pyrene degradation or bacterial growth. The highest pyrene degradation rate in the presence of phenanthrene was consistent with the highest transcription level of nidA and 4,5-pyrenediol as the sole initial metabolite. This study reveals that bacterial growth requirement can invigorate degradation of PAHs by regulating metabolic pathway and genotypic enzyme expression. © 2016 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/87138
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: MOE Key Laboratory of Aquatic Product Safety, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, School of Marine Sciences, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou, China
Recommended Citation:
Chen B.,Huang J.,Yuan K.,et al. Direct evidences on bacterial growth pattern regulating pyrene degradation pathway and genotypic dioxygenase expression[J]. Marine Pollution Bulletin,2016-01-01,105(1)