DOI: 10.1016/j.marpolbul.2017.03.023
Scopus记录号: 2-s2.0-85016047209
论文题名: Identification of heavy metal pollutant tolerance-associated genes in Avicennia marina (Forsk.) by suppression subtractive hybridization
作者: Zhang J. ; Yu J. ; Hong H. ; Liu J. ; Lu H. ; Yan C.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2017
卷: 119, 期: 1 起始页码: 81
结束页码: 91
语种: 英语
英文关键词: Avicennia marina
; Differentially expressed genes
; Heavy metal pollutant tolerance
; Molecular mechanism
; Suppression subtractive hybridization
Scopus关键词: Cadmium
; Genes
; Heavy metals
; Marinas
; Plants (botany)
; Pollution
; Polymerase chain reaction
; Appropriate materials
; Differentially expressed gene
; Heavy metal pollutants
; Heavy metal stress
; Heavy metal tolerance
; Molecular mechanism
; Suppression subtractive hybridization
; Suppressive subtractive hybridizations
; Gene expression
; heavy metal
; heavy metal
; DNA
; gene expression
; heavy metal
; hybridization
; leaf
; mangrove
; pollution tolerance
; polymerase chain reaction
; Article
; atomic absorption spectrometry
; Avicennia marina
; chemical binding
; cold stress
; DNA sequence
; dot hybridization
; down regulation
; environmental exposure
; environmental monitoring
; enzyme activity
; expressed sequence tag
; genetic variability
; germination
; homeostasis
; molecular dynamics
; nonhuman
; osmotic stress
; plant leaf
; plant stress
; pollutant
; protein protein interaction
; quantitative analysis
; real time polymerase chain reaction
; salt stress
; sequence alignment
; sequence homology
; species diversity
; stress
; suppression subtractive hybridization
; Avicennia
; China
; genetics
; plant gene
; subtractive hybridization
; toxicity
; water pollutant
; China
; Avicennia marina
; Rhizophoraceae
; Avicennia
; China
; Genes, Plant
; Metals, Heavy
; Real-Time Polymerase Chain Reaction
; Subtractive Hybridization Techniques
; Water Pollutants
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: The halophytic Avicennia marina (Forsk.) is one of the pioneer mangroves along the south coast of China. It is an appropriate material for understanding molecular mechanisms of heavy metal tolerance in mangrove plants. A forward and a reverse cDNA library was constructed by PCR-based suppressive subtractive hybridization (SSH) to isolate these tolerance-associated genes from A. marina leaves. A total of 99 ESTs obtained from the forward and reverse libraries showed significant differential expressions. Twenty-nine genes selected by SSH were studied by real-time PCR in order to analyze their expression level. Most of these genes' expression increased in leaves under Cd stress, which suggests that these genes contribute to the heavy metal tolerance in A. marina. The diversity of these genes indicated that heavy metal stress resulted in a complex response in mangrove plants. This could prove a useful approach for further exploring the molecular mechanisms behind such heavy metal tolerance. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/87578
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
There are no files associated with this item.
作者单位: Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen, China
Recommended Citation:
Zhang J.,Yu J.,Hong H.,et al. Identification of heavy metal pollutant tolerance-associated genes in Avicennia marina (Forsk.) by suppression subtractive hybridization[J]. Marine Pollution Bulletin,2017-01-01,119(1)