Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要:
Polycyclic aromatic hydrocarbons are very important components of oil pollution. These pollutants tend to sorb to cell surfaces, exerting toxic effects on organisms. Our study developed a flow cytometric method for the detection of PAHs sorbed to phytoplankton by exploiting their spectral characteristics. We discriminated between cells with PAHs from cells free of PAHs. Clear discrimination was observed with flow cytometer provided with 375 or 405�nm lasers in addition to the standard 488�nm laser necessary to identify phytoplankton. Using this method, we measured the relationship between the percentages of phytoplankton organisms with PAHs, with the decrease in the growth rate. Moreover, the development of this method could be extended to facilitate the study of PAHs impact on cell cultures from a large variety of organisms. � 2017 Elsevier Ltd
Department of Global Change Research, IMEDEA (CSIC-UIB), Instituto Mediterr�neo de Estudios Avanzados, Miquel Marqu�s 21, Esporles, Spain; Centre for Microscopy, Characterisation and Analysis (CMCA), The University of Western Australia, Mail Bag 510, 35 Stirling Highway, Crawley, WA, Australia; Red Sea Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
Recommended Citation:
Cerezo M.I.,Linden M.,Agust� S.. Flow cytometry detection of planktonic cells with polycyclic aromatic hydrocarbons sorbed to cell surfaces[J]. Marine Pollution Bulletin,2017-01-01,118(2018-01-02)