Detection of metal induced cytopathological alterations and DNA damage in the gills and hepatopancreas of green mussel Perna viridis from Ennore Estuary, Chennai, India
Biomarkers
; Damage detection
; DNA
; Estuaries
; Heavy metals
; Marine pollution
; Molluscs
; Transmission electron microscopy
; Water pollution
; Coastal waters
; Cytopathology
; DNA damages
; Eco-toxicology
; Electron-dense particles
; Genotoxicology
; Hepatopancreatic cells
; Perna viridis
; Principal component analysis
; DNA
; metal
; water pollutant
; biomarker
; DNA
; estuarine pollution
; heavy metal
; mollusc
; pathology
; ultrastructure
; animal cell
; animal tissue
; Article
; cell compartmentalization
; controlled study
; cytopathology
; DNA damage
; gill
; hepatopancreas
; India
; microvillus
; mitochondrion
; nonhuman
; Perna viridis
; principal component analysis
; sea pollution
; animal
; chemistry
; cytology
; environmental monitoring
; estuary
; gill
; hepatopancreas
; pathology
; Perna
; water pollutant
; Chennai
; Ennore Estuary
; India
; Kovalam Estuary
; Tamil Nadu
; Musculista senhousia
; Perna viridis
; Animals
; DNA Damage
; Environmental Monitoring
; Estuaries
; Gills
; Hepatopancreas
; India
; Perna
; Water Pollutants, Chemical
Scopus学科分类:
Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要:
This study report the impact of heavy metals on cytopathology and DNA damage in the gills and hepatopancreas of Perna viridis collected from Ennore estuary and the Kovalam coastal waters. Principal Component Analysis (PCA) showed significant differences among all variables at the scale of plots. The ultrastructural alterations such as lack of microvilli, distorted mitochondria, electron dense particles and the presence of large mucous droplets were common in the gill and hepatopancreatic cells of mussels from Ennore estuary. However, the gill and hepatopancreatic cells of P. viridis from Kovalam revealed normal compartmentalization of cells. The percentage of tail DNA in the mussels from Ennore estuary was recorded as 12.44 and 10.14% in the gills and hepatopancreas respectively. Overall, it has been demonstrated that the Comet and cytopathological assays are useful biomarkers to assess the level of pollution and it provide reliable information on ecotoxicology and genotoxicology of coastal waters. � 2017 Elsevier Ltd
Department of Environmental Biotechnology, School of Environmental Sciences, Bharathidasan University, Tiruchirappalli, Tamilnadu, India; Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamilnadu, India
Recommended Citation:
Vasanthi L.A.,Revathi P.,Babu Rajendran R.,et al. Detection of metal induced cytopathological alterations and DNA damage in the gills and hepatopancreas of green mussel Perna viridis from Ennore Estuary, Chennai, India[J]. Marine Pollution Bulletin,2017-01-01,117(2018-01-02)