Animals
; Cell death
; DNA
; Gene expression
; Molluscs
; Nucleic acids
; Pollution
; Pyrene
; Repair
; Shellfish
; Benzo [a] pyrene
; DNA damages
; DNA repair
; Scrobicularia plana
; Spermatozoa
; Risk assessment
; benzo[a]pyrene
; caspase 3
; caspase 7
; cycline
; messenger RNA
; caspase 3
; cycline
; water pollutant
; apoptosis
; assay
; bivalve
; DNA
; estuarine pollution
; gene expression
; genotoxicity
; PAH
; sperm
; animal cell
; apoptosis
; Article
; bivalve
; cell viability
; comet assay
; concentration (parameters)
; controlled study
; DNA repair
; DNA strand breakage
; enzyme activity
; gene expression
; genotoxicity
; male
; nonhuman
; protein expression
; Scrobicularia plana
; spermatozoon
; animal
; bivalve
; DNA damage
; drug effects
; metabolism
; spermatozoon
; toxicity
; water pollutant
; Animalia
; Bivalvia
; Scrobicularia plana
; Animals
; Apoptosis
; Benzo(a)pyrene
; Bivalvia
; Caspase 3
; Comet Assay
; DNA Damage
; Male
; Proliferating Cell Nuclear Antigen
; Spermatozoa
; Water Pollutants, Chemical
Scopus学科分类:
Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要:
Evaluation of DNA quality of gametes is a relevant method to predict potential consequences of pollutants in the next generations, as it allows to define adverse outcome pathways implicated in pollutant-mediated toxicity for risk assessment. In the present study, a comet assay was developed for the spermatozoa of Scrobicularia plana exposed to 10 and 100�μg/L of benzo[a]pyrene (B[a]P) for 24�h and 5�days. The induction of apoptosis and repair mechanisms was assessed by determining caspase-3 activity and polymerase cell nuclear antigen (PCNA) mRNA expression level. Results showed that B[a]P induced high levels of DNA breaks that were associated with apoptosis for all the conditions tested, indicating that the spermatozoa were sensitive to B[a]P. PCNA gene expression was induced in animals exposed to the highest concentrations of B[a]P, suggesting that defence mechanisms were enhanced in these animals. This preliminary study demonstrated the utility of spermatozoa as a relevant biological target for genotoxicity assessment of contaminants and will enable to predict the effect of contaminants on future generations. � 2016 Elsevier Ltd
Laboratoire Mer, Mol�cules, Sant� (MMS, EA 2160), Universit� Catholique de l'Ouest, Angers, France
Recommended Citation:
Ch�tel A.,Bruneau M.,Li�vre C.,et al. Spermatozoa: A relevant biological target for genotoxicity assessment of contaminants in the estuarine bivalve Scrobicularia plana[J]. Marine Pollution Bulletin,2017-01-01,116(2018-01-02)