DOI: 10.1016/j.marpolbul.2017.06.066
Scopus记录号: 2-s2.0-85021066860
论文题名: Selective isolation of gonyautoxins 1,4 from the dinoflagellate Alexandrium minutum based on molecularly imprinted solid-phase extraction
作者: Lian Z. ; Wang J.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2017
卷: 122, 期: 2018-01-02 起始页码: 500
结束页码: 504
语种: 英语
英文关键词: Alexandrium minutum
; Double-templated imprinting
; Gonyautoxins 1,4
; Molecularly imprinted solid-phase extraction
Scopus关键词: Phase separation
; Polymers
; Alexandrium minutum
; Extraction efficiencies
; Extraction procedure
; Gonyautoxins 1,4
; Molecular imprinting technology
; Molecularly Imprinted Polymer
; Molecularly imprinted solid-phase extraction
; Templated
; Extraction
; caffeine
; ethylene glycol dimethacrylate
; gonyautoxin
; gonyautoxin 1,4
; gonyautoxin 2,3
; methacrylic acid
; microsphere
; pentoxifylline
; polymer microsphere
; polyvinyl alcohol
; sorbent
; unclassified drug
; gonyautoxins
; polymer
; saxitoxin
; dinoflagellate
; extraction method
; microalga
; polymer
; toxin
; adsorption
; Alexandrium
; Alexandrium minutum
; Article
; binding affinity
; chemical structure
; concentration (parameters)
; controlled study
; high performance liquid chromatography
; high performance liquid chromatography fluorescence detection
; hydrogen bond
; isolation procedure
; measurement repeatability
; molecular imprinting
; molecular recognition
; nonhuman
; polymerization
; reproducibility
; solid phase extraction
; surface area
; suspension polymerization
; synthesis
; analogs and derivatives
; dinoflagellate
; solid phase extraction
; Alexandrium minutum
; Dinophyceae
; Chromatography, High Pressure Liquid
; Dinoflagellida
; Molecular Imprinting
; Polymers
; Saxitoxin
; Solid Phase Extraction
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Gonyautoxins 1,4 (GTX1,4) from Alexandrium minutum samples were isolated selectively and recognized specifically by an innovative and effective extraction procedure based on molecular imprinting technology. Novel molecularly imprinted polymer microspheres (MIPMs) were prepared by double-templated imprinting strategy using caffeine and pentoxifylline as dummy templates. The synthesized polymers displayed good affinity to GTX1,4 and were applied as sorbents. Further, an off-line molecularly imprinted solid-phase extraction (MISPE) protocol was optimized and an effective approach based on the MISPE coupled with HPLC-FLD was developed for selective isolation of GTX1,4 from the cultured A. minutum samples. The separation method showed good extraction efficiency (73.2–81.5%) for GTX1,4 and efficient removal of interferences matrices was also achieved after the MISPE process for the microalgal samples. The outcome demonstrated the superiority and great potential of the MISPE procedure for direct separation of GTX1,4 from marine microalgal extracts. © 2017
Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/88123
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
There are no files associated with this item.
作者单位: Marine College, Shandong University, Weihai, China; Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, China
Recommended Citation:
Lian Z.,Wang J.. Selective isolation of gonyautoxins 1,4 from the dinoflagellate Alexandrium minutum based on molecularly imprinted solid-phase extraction[J]. Marine Pollution Bulletin,2017-01-01,122(2018-01-02)