DOI: 10.1016/j.marpolbul.2018.02.034
Scopus记录号: 2-s2.0-85042857571
论文题名: Microbial community and metagenome dynamics during biodegradation of dispersed oil reveals potential key-players in cold Norwegian seawater
作者: Ribicic D. ; Netzer R. ; Hazen T.C. ; Techtmann S.M. ; Drabløs F. ; Brakstad O.G.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2018
卷: 129, 期: 1 起始页码: 370
结束页码: 378
语种: 英语
英文关键词: 16S rRNA
; Biodegradation
; Bioremediation
; Chemical dispersant
; Metagenomics
; Petroleum
Scopus关键词: Bioremediation
; Crude oil
; Genes
; Hydrocarbons
; Microorganisms
; Paraffins
; RNA
; Seawater
; Weathering
; 16S rRNA
; Biodegradation process
; Dispersants
; Hydrocarbon biodegradation
; Metagenomics
; Microbial communities
; Microcosm experiments
; Weathering process
; Biodegradation
; alkane
; oil
; polycyclic aromatic compound
; RNA 16S
; sea water
; biodegradation
; bioremediation
; enzyme
; gene
; genomics
; microbial community
; oil spill
; petroleum
; RNA
; seawater
; Article
; bacterial gene
; bacterial genome
; bacterium
; Bermanella
; bioremediation
; Colwellia
; controlled study
; metagenome
; microbial community
; microbial degradation
; nonhuman
; Norway
; Porticoccaceae
; Spongiiabcteraceae
; Zhongshania
; Norway
; Bacteria (microorganisms)
; Colwellia
; Cycloclasticus
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Oil biodegradation as a weathering process has been extensively investigated over the years, especially after the Deepwater Horizon blowout. In this study, we performed microcosm experiments at 5 °C with chemically dispersed oil in non-amended seawater. We link biodegradation processes with microbial community and metagenome dynamics and explain the succession based on substrate specialization. Reconstructed genomes and 16S rRNA gene analysis revealed that Bermanella and Zhongshania were the main contributors to initial n-alkane breakdown, while subsequent abundances of Colwellia and microorganisms closely related to Porticoccaceae were involved in secondary n‑alkane breakdown and beta‑oxidation. Cycloclasticus, Porticoccaceae and Spongiiabcteraceae were associated with degradation of mono- and poly-cyclic aromatics. Successional pattern of genes coding for hydrocarbon degrading enzymes at metagenome level, and reconstructed genomic content, revealed a high differentiation of bacteria involved in hydrocarbon biodegradation. A cooperation among oil degrading microorganisms is thus needed for the complete substrate transformation. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/88396
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: NTNU Norwegian University of Science and Technology, Department of Clinical and Molecular Medicine, Trondheim, Norway; SINTEF OCEAN, Trondheim, Norway; University of Tennessee Knoxville, Department of Civil and Environmental Engineering, Knoxville, TN, United States; Michigan Technological University, Department of Biological Sciences, Houghton, MI, United States
Recommended Citation:
Ribicic D.,Netzer R.,Hazen T.C.,et al. Microbial community and metagenome dynamics during biodegradation of dispersed oil reveals potential key-players in cold Norwegian seawater[J]. Marine Pollution Bulletin,2018-01-01,129(1)