项目编号: | 1748695
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项目名称: | EAGER: SusChEM: Developing Temporal Compartmentalization Tools in Yeast to Enhance the Bioproduction of Phytosterols |
作者: | Yanran Li
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承担单位: | University of California-Riverside
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批准年: | 2017
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开始日期: | 2017-10-01
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结束日期: | 2018-09-30
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资助金额: | 100000
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资助来源: | US-NSF
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项目类别: | Standard Grant
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国家: | US
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语种: | 英语
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特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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英文关键词: | yeast
; temporal regulation strategy
; research project
; yeast endogenous temporal compartmentalization strategy
; engineer yeast
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英文摘要: | Plant steroids are complex, naturally occurring molecules that have a wide variety of pharmaceutical and agricultural applications; however, they are usually found in quite small amounts in plants, and they are difficult to synthesize chemically. This research project investigates ways to engineer yeasts to produce these molecules. To accomplish this goal, it is necessary to introduce into the yeast the ability to perform the targeted degradation of specific proteins. The researchers on this project are employing advanced biochemical methods in order to realize this alteration in the yeast. This research project include specific activities for high school students and for undergraduate students from a local community college, which will help in developing a highly trained technical workforce for the biotechnology industry.
This research project provides a foundation for the development of programmable temporal regulation machinery for complex heterologous plant pathways in yeast. Such machinery employs yeast endogenous temporal compartmentalization strategies and an inducible protein degradation system associated with the endoplasmic reticulum (ER). Introducing complex plant pathways into microbial hosts including yeast has been highly challenging, mainly due to 1) the high burden from the expression of large numbers of plant enzymes; and 2) the lack of efficient coordination among the many heterologous enzymatic steps. A plant biosynthetic platform is required to incorporate and validate the proposed temporal regulation strategy, as is a targeted protein degradation (TPD) approach. The biosynthetic pathway of the plant steroid hormone brassinosteroids (BRs) is being verified and established in yeast, which will enable the downstream establishment of the proposed temporal regulation strategies. In addition, the feasibility of introducing TPD in the proposed temporal regulation strategy is being evaluated. |
资源类型: | 项目
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标识符: | http://119.78.100.158/handle/2HF3EXSE/88711
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Appears in Collections: | 全球变化的国际研究计划 科学计划与规划
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Recommended Citation: |
Yanran Li. EAGER: SusChEM: Developing Temporal Compartmentalization Tools in Yeast to Enhance the Bioproduction of Phytosterols. 2017-01-01.
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