globalchange  > 全球变化的国际研究计划
Project number: 1748695
EAGER: SusChEM: Developing Temporal Compartmentalization Tools in Yeast to Enhance the Bioproduction of Phytosterols
Author: Yanran Li
Publisher: University of California-Riverside
Publishing Year: 2017
Start date of project: 2017-10-01
End date of project: 2018-09-30
Amount: 100000
grant: US-NSF
Project Type: Standard Grant
Country Filed: US
Language: 英语
Subject of Source: Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
Keyword: yeast ; temporal regulation strategy ; research project ; yeast endogenous temporal compartmentalization strategy ; engineer yeast
English Abstract: Plant steroids are complex, naturally occurring molecules that have a wide variety of pharmaceutical and agricultural applications; however, they are usually found in quite small amounts in plants, and they are difficult to synthesize chemically. This research project investigates ways to engineer yeasts to produce these molecules. To accomplish this goal, it is necessary to introduce into the yeast the ability to perform the targeted degradation of specific proteins. The researchers on this project are employing advanced biochemical methods in order to realize this alteration in the yeast. This research project include specific activities for high school students and for undergraduate students from a local community college, which will help in developing a highly trained technical workforce for the biotechnology industry.

This research project provides a foundation for the development of programmable temporal regulation machinery for complex heterologous plant pathways in yeast. Such machinery employs yeast endogenous temporal compartmentalization strategies and an inducible protein degradation system associated with the endoplasmic reticulum (ER). Introducing complex plant pathways into microbial hosts including yeast has been highly challenging, mainly due to 1) the high burden from the expression of large numbers of plant enzymes; and 2) the lack of efficient coordination among the many heterologous enzymatic steps. A plant biosynthetic platform is required to incorporate and validate the proposed temporal regulation strategy, as is a targeted protein degradation (TPD) approach. The biosynthetic pathway of the plant steroid hormone brassinosteroids (BRs) is being verified and established in yeast, which will enable the downstream establishment of the proposed temporal regulation strategies. In addition, the feasibility of introducing TPD in the proposed temporal regulation strategy is being evaluated.
Document Type: 项目
Appears in Collections:全球变化的国际研究计划

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Yanran Li. EAGER: SusChEM: Developing Temporal Compartmentalization Tools in Yeast to Enhance the Bioproduction of Phytosterols. 2017-01-01.
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