| 项目编号: | 1706916
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| 项目名称: | Illuminating organelle dynamics through highly scattering bone: intravital wavefront optimized structured illumination imaging |
| 作者: | Luke Mortensen
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| 承担单位: | University of Georgia Research Foundation Inc
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| 批准年: | 2017
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| 开始日期: | 2017-07-01
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| 结束日期: | 2020-06-30
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| 资助金额: | 501655
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | deep tissue imaging
; illumination imaging
; optical imaging
; 2-photon
; mitochondria dynamics
; mouse bone
; super-resolution structured illumination microscopy
; intravital imaging
; bone marrow
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| 英文摘要: | Mortensen 1706916
This proposal describes a range of optical designs and engineering tasks to create an optimized microscope for use in deep tissue imaging. If successful, the proposed work will extend super-resolution structured illumination microscopy substantially and will provide a significant biological advance for tracking and monitoring engineered stem cells in deep tissues. In vivo optical imaging at depth with cellular level resolution is a major challenge to the clinical translation of biophotonic technology and the proposed resolution and depth of penetration metrics would be very beneficial to the field. The PIs support a diverse group of trainees (including high school students) and will develop two new courses on microscopy for biologists and biology for microscopists.
The proposed research will benefit a range of fields including the skeletal biology, hematopoietic and neuroscience communities. To enable sub-diffraction microscopy in living bone marrow, three objectives will be pursued: 1.) A dedicated 2-photon microscope will be designed and built with a deformable mirror and digital micromirror device. 2.) The wavefront shaping 2-photon scanning microscope will be enhanced for live 2-photon structured illumination imaging resulting in 150nm lateral resolution. 3.) The instrument will be tested and validated by intravital imaging of mitochondria dynamics in Mesenchymal Stem Cells (MSCs) in mouse bone. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/89947
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Luke Mortensen. Illuminating organelle dynamics through highly scattering bone: intravital wavefront optimized structured illumination imaging. 2017-01-01.
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