| 项目编号: | 1653782
|
| 项目名称: | CAREER: Single-molecule imaging of T cell recognition and signaling |
| 作者: | Jun Huang
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| 承担单位: | University of Chicago
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| 批准年: | 2017
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| 开始日期: | 2017-02-15
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| 结束日期: | 2022-01-31
|
| 资助金额: | 500000
|
| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
|
| 国家: | US
|
| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
|
| 英文关键词: | t-cell
; signaling
; student
; 1653782t-cell
; t cell recognition
; dynamic interaction
; educational material
; single-molecule resonance energy transfer
; cellular abnormality
; cell membrane
; t-cell recognition
; proximal tcr signal transduction
; antigen recognition
; web tutorial
; t-cell receptor conformational change
; t-cell study
; early t cell intracellular signaling kinetics
; novel technology
; light-sheet microscopy
; membrane receptor
; t-cell receptor
; white blood cell
; resonance energy transfer
; enough spatiotemporal resolution
; histocompatibility complex
; t cell
; research-intensive learning environment
; pi
; signaling event
; fluorescent marker
; measure early t-cell intracellular signaling kinetics
; research project
|
| 英文摘要: | Abstract
Huang; 1653782
T-cells are a type of white blood cell that circulate around our bodies, scanning for cellular abnormalities and infections. The research goal is to understand the molecular mechanism of T cell recognition and signaling, which determines the selection, development, fate, and function of a T cell. Existing T-cell studies cannot directly visualize and measure the dynamic interactions and signaling events with enough spatiotemporal resolution. The PI proposes to use single-molecule resonance energy transfer and light-sheet microscopy to address this immunology problem by measuring early T cell intracellular signaling kinetics.
The PI will integrate research within teaching and create a research-intensive learning environment to educate students at the interface of immunology and engineering through a new emerging discipline called immune-engineering. In addition, the PI will develop educational materials, demonstrations, and web tutorials to inspire students to explore new research areas, and develop novel technologies to drive the booming field of immune-engineering.
The research project will use Forster's resonance energy transfer (FRET) to study molecular mechanisms of T cell recognition and signaling. The PI will attach fluorescent markers to the membrane receptors of T cells responsible for recognition and signaling. Furthermore, the PI will visualize T-cell receptor conformational changes, quantify the role of CD4, and measure early T-cell intracellular signaling kinetics in-situ. Also, he will observe T-cell receptor (TCR) and CD4 binding to peptide bound histocompatibility complexes. This initiates T-cell recognition and signaling at the cell membrane; antigen recognition will trigger proximal TCR signal transduction. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/90527
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
|
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| Recommended Citation: | |
Jun Huang. CAREER: Single-molecule imaging of T cell recognition and signaling. 2017-01-01.
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