| 项目编号: | 1604728
|
| 项目名称: | Engineering Nonphosphorylative Metabolism for Biosynthesis |
| 作者: | Kechun Zhang
|
| 承担单位: | University of Minnesota-Twin Cities
|
| 批准年: | 2016
|
| 开始日期: | 2016-07-01
|
| 结束日期: | 2019-06-30
|
| 资助金额: | 311587
|
| 资助来源: | US-NSF
|
| 项目类别: | Standard Grant
|
| 国家: | US
|
| 语种: | 英语
|
| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
|
| 英文关键词: | nonphosphorylative metabolism
; metabolic engineering technique
; efficient biosynthesis
; non-phosphorylative metabolism
; biochemical engineering program
; new non-phosphorylative metabolism
|
| 英文摘要: | 1604728
Zhang, Kechun
Traditionally, glycolysis and the pentose phosphate pathway are the standard metabolic routes for sugar assimilation. However, their lengthy reaction steps and complex regulation limit yield and production rate of a variety of bioproducts. The objective of this proposal is to apply metabolic engineering techniques to implement a pathway for the direct conversion of sugars into intermediates of the citric acid cycle. This will improve the carbon yield of a variety of products and improve their manufacturing processes.
With higher theoretical carbon yield and shorter reaction routes than the conventional glycolysis and pentose phosphate pathways, this new non-phosphorylative metabolism has the potential for efficient biosynthesis of citric acid cycle derivatives. Although this pathway has been known for more than fifty years, the relevant enzymes remain largely unknown and uncharacterized. To enable the implementation of nonphosphorylative metabolism as a general biosynthetic platform, three specific aims will be pursued: (i) discover and evolve new gene clusters for nonphosphorylative metabolism; (ii) explore biosynthetic applications of non-phosphorylative metabolism; and (iii) co-utilize lignocellulosic feedstocks for biosynthesis. The successful realization of this project is expected to result in the fundamental understanding of a novel catabolic pathway which promises to be more efficient in many practical applications. In addition, this project will enable the processing of non-food agricultural byproducts, such as corn stover, wheat straw and citrus peel, into high-value products. The proposed new pathway will enhance the economic viability of cellulosic biorefineries which would be of significant social, environmental, and economic value.
This award by the Biotechnology and Biochemical Engineering Program of the CBET Division is co-funded by the Systems and Synthetic Biology Program of the Division of Molecular and Cellular Biosciences. |
| 资源类型: | 项目
|
| 标识符: | http://119.78.100.158/handle/2HF3EXSE/91950
|
| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
|
|
There are no files associated with this item.
|
| Recommended Citation: | |
Kechun Zhang. Engineering Nonphosphorylative Metabolism for Biosynthesis. 2016-01-01.
|
|
|