| 项目编号: | 1437836
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| 项目名称: | Enhanced Production of Advanced Biofuels through Model Guided Synthetic Biology |
| 作者: | Mark Blenner
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| 承担单位: | Clemson University
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| 批准年: | 2014
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| 开始日期: | 2015-01-01
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| 结束日期: | 2018-12-31
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| 资助金额: | USD336751
|
| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
|
| 英文关键词: | stem
; biodiesel production
; synthetic biology
; research
; simple unsteady-state kinetic model
; project
; preliminary modeling study
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| 英文摘要: | Principal Investigator: Mark Blenner
Number: 1437836
This project seeks to improve the production of biodiesel in a genetically engineered strain of bacteria by understanding how sugars are converted into biodiesel. This will be accomplished through cell-based monitoring of the microorganism?s metabolism control processes through the principles of synthetic biology, which look holistically at how all metabolic processes within a cell interact with one another. A second aim of this project is control these metabolic processes to maintain high biodiesel production rates under changing environmental or process conditions. The results of this project may also apply to other metabolic pathways that produce sustainable fuels and chemicals, allowing transfer of these techniques from the laboratory to industry with more certainty and fewer complications. The project activities will also include efforts to engage women potentially interested in science, technology, engineering, and mathematics (STEM) careers in the state of South Carolina. Education and research will be integrated by providing training opportunities for students already in STEM, and local outreach to young women who might become more interested in STEM. Finally, this research will be integrated into a new elective course on protein and metabolic engineering offered at Clemson University.
Technical Description
This project will use simple unsteady-state kinetic models to rationalize protein engineering and synthetic biology based improvements to E. coli biodiesel production. Preliminary modeling studies identify inefficiencies due to pathway imbalance, and suggest that better flux distributions can be achieved through genetic and protein engineering efforts. Enzymes with catalytic efficiencies predicted to improve biodiesel production will be used. Critical enzymes will be engineered using directed evolution to relieve pathway bottlenecks. Protein-level feedback control will be engineered and combined with genetic level feedback control to allow pathway fluxes to remain high in spite of short and long time-scale metabolic perturbations. The combination of genetic and protein-level dynamic control will allow biofuel and other chemical producing systems to withstand perturbations from environmental variation and scale-up conditions without suffering large losses in yield and efficiency. The results of this project may lead to more general methods for balancing pathways that does not rely on explicit kinetic or flux data. The project activities will also include efforts to engage women potentially interested in science, technology, engineering, and mathematics (STEM) careers in the state of South Carolina. Education and research will be integrated by providing training opportunities for students already in STEM, and local outreach to young women who might become more interested in STEM. Finally, this research will be integrated into a new elective course on protein and metabolic engineering offered at Clemson University. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/95281
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Mark Blenner. Enhanced Production of Advanced Biofuels through Model Guided Synthetic Biology. 2014-01-01.
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