| 项目编号: | 1450936
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| 项目名称: | BRAIN EAGER: New Tools for Real-Time Imaging of Molecular-Resolution Connectomics of Synapses |
| 作者: | X. Nancy Xu
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| 承担单位: | Old Dominion University Research Foundation
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| 批准年: | 2013
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| 开始日期: | 2014-09-01
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| 结束日期: | 2018-08-31
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| 资助金额: | USD359307
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | function
; role
; real-time
; real time
; powerful new tool
; brain eager
; synapsessignificancethe successful outcome
; new insight
; novel imaging platform
; brain function
; molecular resolution
; real-time imaging
; real-time characterization
; molecular machinery
; neurotransmitter
; molecular-resolution connectomics
; eager project
; temporal resolution level
; technical descriptionthe brain
; related molecular pathway
; molecular dynamics
; new tools
; molecular resolution level
; molecular identification
; temporal resolution
|
| 英文摘要: | PI: Xu, X. Nancy
Proposal: 1450936
Title: BRAIN EAGER: New Tools for Real-Time Imaging of Molecular-Resolution Connectomics of Synapses
Significance
The successful outcome of this EAGER project will have a broad impact in neuroscience. The proposed project will offer new insights into the roles and functions of neurotransmitters in synaptic plasticity and regeneration. This will lead to the development of innovative tools for molecular identification and characterization of roles and functions of multiple types of neurotransmitters, their receptors, and their interactions and dynamics in synapses at the spatial and temporal resolution level. These powerful new tools are expected to become extremely valuable in addressing a wide range of pressing biological, biochemical and biomedical questions related to molecular and real-time characterization of functions of single live cells (neurons).
Technical Description
The brain comprises large number of neurons, which are not continuous. Synapses enable them to communicate complex and specific commands with each other by passing specific electrical or chemical signals to another cell. Each synapse contains extensive arrays of molecular machinery that links the membranes of the coupled partner neurons, an array of neurotransmitters and their receptors. The aims of this project is to develop a novel imaging platform, including next-generation multicolored far-field photostable optical nanoscopy (PHOTON) with photostable multicolored single molecule nanoparticle optical biosensors (SMNOBS). This platform will quantitatively image and molecularly characterize roles and functions of multiple types of molecules (neurotransmitters, receptors) at individual live synapses in real time at nanometer (nm) resolution. These capabilities will enable the identification of the roles and functions of single neurotransmitter-receptor interactions, identify and characterize their roles in modulating and regulating functions of single synapses and neurons in real time. They will use the multicolored PHOTON: (i) to quantitatively and simultaneously detect and map diffusion and molecular dynamics of multiple types of neurotransmitters, their receptors and their interactions on each synapse with both spatial and temporal resolutions; (ii) to construct the connection of individual neurons at individual synapses at the molecular resolution; (iii) to explore the possibility of creating connectomics to determine the functions and related molecular pathways of synapses and their roles in neuron and brain functions at the molecular resolution level. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/95530
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
X. Nancy Xu. BRAIN EAGER: New Tools for Real-Time Imaging of Molecular-Resolution Connectomics of Synapses. 2013-01-01.
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