| 项目编号: | 1405103
|
| 项目名称: | DISSERTATION RESEARCH: Divergence of long intergenic noncoding RNAs in Drosophila pseudoobscura, Drosophila persimilis, and their hybrids |
| 作者: | Carlos Machado
|
| 承担单位: | University of Maryland College Park
|
| 批准年: | 2013
|
| 开始日期: | 2014-06-01
|
| 结束日期: | 2016-05-31
|
| 资助金额: | USD19553
|
| 资助来源: | US-NSF
|
| 项目类别: | Standard Grant
|
| 国家: | US
|
| 语种: | 英语
|
| 特色学科分类: | Biological Sciences - Environmental Biology
|
| 英文关键词: | rna-seq
; hybrid
; lincrna expression
; lincrna
; long intergenic non-coding rna
; dissertation improvement grant
; lincrna regulation
; summer research experience
; evolution
; next generation rna sequencing technology
; lincrna regulatory divergence
; lincrna evolution
; lincrna regulatory evolution
; persimilis hybrid
; lincrna sequence
; hybrid incompatibility
|
| 英文摘要: | Long intergenic non-coding RNAs (lincRNAs) are a recently discovered group of transcripts that have no protein-coding capacity. Although they play diverse and important roles in the biology of complex organisms, almost nothing is known of their origin and evolution. The goal of this project is to use next generation RNA sequencing technology (RNA-Seq) and the vast genomic resources of the fruit fly Drosophila to characterize the forces that underlie lincRNA sequence and expression evolution. Analyses of variation in pure species cannot explain how the regulation of lincRNA expression evolves, but analyses of misexpression in hybrids provides insight into how diverse evolutionary processes shape the regulatory process. Thus, the specific goal of this dissertation improvement grant is to analyze the evolution of lincRNA regulation using RNA-Seq in D. pseudoobscura/D. persimilis hybrids. Specific aims are (1) to identify the mode of inheritance of lincRNA expression, (2) identify whether lincRNA regulatory divergence occurs through cis or trans changes, and (3) identify whether lincRNAs may underlie hybrid incompatibilities.
This work will be the most comprehensive analysis of lincRNA evolution to date and the first to examine lincRNA regulatory evolution in closely related species via hybrids. It will underscore the importance of an understudied class of genes that deviates from the central dogma of biology. This grant will support the training of both a doctoral student and multiple undergraduates as well as provide a summer research experience for a local high school student or teacher from the University of Maryland's Minority Student Pipeline. The knowledge gleaned from this work will be disseminated widely, both in national presentations and in print. Understanding the evolution of gene regulatory systems provides important insights into the relationship between genotype and phenotype, which ultimately underlies our understanding of disease and the improvement of agricultural crops. |
| 资源类型: | 项目
|
| 标识符: | http://119.78.100.158/handle/2HF3EXSE/96751
|
| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
|
|
There are no files associated with this item.
|
| Recommended Citation: | |
Carlos Machado. DISSERTATION RESEARCH: Divergence of long intergenic noncoding RNAs in Drosophila pseudoobscura, Drosophila persimilis, and their hybrids. 2013-01-01.
|
|
|