| 项目编号: | 1405665
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| 项目名称: | Dissertation Research: Systematics and evolutionary history of the southern redback salamander: using next generation sequencing with organisms with large genomes |
| 作者: | Christopher Austin
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| 承担单位: | Louisiana State University & Agricultural and Mechanical College
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| 批准年: | 2013
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| 开始日期: | 2014-06-01
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| 结束日期: | 2018-05-31
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| 资助金额: | USD19240
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Biological Sciences - Environmental Biology
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| 英文关键词: | large genome
; salamander
; dna sequencing
; genome-wide data set
; dna sequencing protocol
; amphibian systematic
; new dna sequencing method
; systematics study
; species
; southern redback salamander
; next-generation dna sequencing technique
; salamander conservation
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| 英文摘要: | The southeastern United States is a hotspot for amphibian biodiversity, but many amphibian species in the region are undergoing rapid declines. This is especially true for salamanders, which are incredibly diverse in the Southeast. Examining genetic relationships, climate factors, and changes in geographic distributions can increase our understanding of the complex mechanisms influencing the generation and decline of species. The goals of this project are to combine data from new methods of DNA sequencing with geographic and environmental data, in order to test hypotheses about evolutionary relationships of the southern redback salamander and changes in its geographic distribution through time.
Salamanders have genomes approximately 10 times larger than humans, and this large genome size can pose problems for new DNA sequencing methods. This project will test the utility of a newly established DNA sequencing protocol in resolving evolutionary relationships for species with large genomes. This research will be the first study to use a next-generation DNA sequencing technique to generate a genome-wide data set of 400-800 million DNA sequence fragments for a species. Results from this study will inform salamander conservation, amphibian systematics, and evolutionary biogeography more generally, as well as the future of systematics studies of organisms with large genomes. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/96794
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Christopher Austin. Dissertation Research: Systematics and evolutionary history of the southern redback salamander: using next generation sequencing with organisms with large genomes. 2013-01-01.
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