DOI: 10.1016/j.watres.2018.08.066
Scopus记录号: 2-s2.0-85053811071
论文题名: The more important role of archaea than bacteria in nitrification of wastewater treatment plants in cold season despite their numerical relationships
作者: Pan K.-L. ; Gao J.-F. ; Fan X.-Y. ; Li D.-C. ; Dai H.-H.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 145 起始页码: 552
结束页码: 561
语种: 英语
英文关键词: Ammonia-oxidizing archaea
; Ammonia-oxidizing bacteria
; Complete ammonia-oxidizing nitrospira
; DNA-based stable isotope probing
; Numerical relationships
; Wastewater treatment plants
Scopus关键词: Ammonia
; Bacteria
; DNA
; Gene encoding
; Isotopes
; Nitrification
; Reclamation
; Sewage pumping plants
; Water treatment plants
; Ammonia oxidizing bacteria
; Ammonia-oxidizing archaea
; Nitrospira
; Numerical relationships
; Stable-isotope probing
; Wastewater treatment plants
; Wastewater treatment
; Bacteria (microorganisms)
; Nitrosomonadales
; Nitrosomonas europaea
; Nitrosomonas oligotropha
; Nitrospira
英文摘要: Nitrification failure of wastewater treatment plants (WWTPs) in cold season calls into investigations of the functional ammonia-oxidizing microorganisms (AOMs). In this study, we report the abundance of ammonia-oxidizing archaea (AOA), bacteria (AOB) and complete ammonia-oxidizing (comammox) Nitrospira in 23 municipal WWTPs in cold season, and explore the correlations between AOMs abundance and their relative contribution to nitrification. The copy numbers of AOA and AOB amoA gene ranged from 2.42 × 107 to 2.47 × 109 and 5.54 × 106 to 3.31 × 109 copies/g sludge, respectively. The abundance of amoA gene of Candidatus Nitrospira inopinata, an important strain of comammox Nitrospira, was stable with averaged abundance of 8.47 × 106 copies/g sludge. DNA-based stable isotope probing (DNA-SIP) assays were conducted with three typical WWTPs in which the abundance of AOA was lower than, similar to and higher than that of AOB, respectively. The results showed that considerable 13C-assimilation by AOA was detected during active nitrification in all WWTPs, whereas just a much lesser extent of 13C-incorporation by AOB and comammox Nitrospira was found in one WWTP. High-throughput sequencing with 13C-labeled DNA also showed the higher reads abundance of AOA than AOB and comammox Nitrospira. Nitrososphaera viennensis was the dominant active AOA, while Nitrosomonas oligotropha and Nitrosomonas europaea were identified as active AOB. The results obtained suggest that AOA, rather than AOB and comammox Nitrospira, dominate ammonia oxidation in WWTPs in cold season despite the numerical relationships of AOMs. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112454
Appears in Collections: 气候减缓与适应
There are no files associated with this item.
作者单位: National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing, 100124, China
Recommended Citation:
Pan K.-L.,Gao J.-F.,Fan X.-Y.,et al. The more important role of archaea than bacteria in nitrification of wastewater treatment plants in cold season despite their numerical relationships[J]. Water Research,2018-01-01,145