DOI: 10.1016/j.watres.2018.07.023
Scopus记录号: 2-s2.0-85049979487
论文题名: Persistence of infectious Enterovirus within free-living amoebae – A novel waterborne risk pathway?
作者: Atanasova N.D. ; Dey R. ; Scott C. ; Li Q. ; Pang X.-L. ; Ashbolt N.J.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 144 起始页码: 204
结束页码: 214
语种: 英语
英文关键词: Enterovirus
; Expelled vesicles
; Free-living amoebae
; Virus persistence and dispersion
Scopus关键词: Bacteria
; Cell culture
; Dispersions
; Viruses
; Water pollution
; Water supply systems
; Engineered water systems
; Enterovirus
; Environmental persistences
; Expelled vesicles
; Free livings
; Gastrointestinal tract
; Pathogenic bacterium
; Water distributions
; Protozoa
; bacterium
; cell organelle
; dispersion
; pathogen
; persistence
; protozoan
; risk
; symptom
; virus
; vulnerability
; amoeba (life cycle stage)
; Article
; coculture
; controlled study
; Coxsackievirus B5
; Enterovirus
; free living amoeba
; nonhuman
; persistent virus infection
; priority journal
; reverse transcription polymerase chain reaction
; transmission electron microscopy
; Vermamoeba vermiformis
; virion
; virus detection
; virus entry
; virus infectivity
; virus replication
; water supply
; Amoeba (genus)
; Coxsackievirus
; Enterovirus
; Protozoa
英文摘要: Free-living amoebae (FLA) are phagocytic protozoa found in natural and engineered water systems. They can form disinfectant-resistant cysts, which can harbor various human pathogenic bacteria, therefore providing them with a means of environmental persistence and dispersion through water distribution and other engineered water systems. The association of FLA with human viruses has been raised, but the limited data on the persistence of infectious virions within amoebae leaves this aspect unresolved. Enteroviruses can cause a wide range of illness and replicate in human respiratory and gastrointestinal tracts, both of which could be exposed through contact with contaminated waters if virus detection and removal are compromised by virion internalization in free-living protozoa. This is especially problematic for high-risk contaminants, such as coxsackieviruses, representative members of the Enterovirus genus that are likely infectious at low doses and cause a variety of symptoms to a vulnerable portion of the population (particularly infants). To investigate Enterovirus persistence within free-living amoebae we co-cultured an infectious clinical coxsackievirus B5 (CVB5) isolate, with the commonly reported tap water amoeba Vermamoeba vermiformis, after which we tracked virus localization and persistence in co-culture over time through a combination of advanced imaging, molecular and cell culture assays. Our results clearly demonstrate that infectious CVB5 can persist in all life stages of the amoebae without causing any visible injury to them. We also demonstrated that the amoeba generated vesicles containing virions that were expelled into the bulk liquid surroundings, a finding previously described for FLA-bacteria interactions, but not for FLA and human pathogenic viruses. Therefore, our findings suggest that the ability of CVB5 to persist in V. vermiformis could be a novel waterborne risk pathway for the persistence and dispersion of infectious human enteric viruses through water systems. © 2018 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112520
Appears in Collections: 气候减缓与适应
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作者单位: Dept. Medical Microbiology and Immunology, University of Alberta, 6-020 Katz Group Centre, Edmonton, AB T6G 2E1, Canada; School of Public Health, Canada University of Alberta, Edmonton, Room 3-57D, South Academic Building, Edmonton, AB T6G 2G7, Canada; Provincial Laboratory for Public Health, Edmonton, AB T6G 2J2, Canada
Recommended Citation:
Atanasova N.D.,Dey R.,Scott C.,et al. Persistence of infectious Enterovirus within free-living amoebae – A novel waterborne risk pathway?[J]. Water Research,2018-01-01,144