DOI: 10.1016/j.watres.2018.12.049
Scopus记录号: 2-s2.0-85059955693
论文题名: What you extract is what you see: Optimising the preparation of water and wastewater samples for in vitro bioassays
作者: Abbas A. ; Schneider I. ; Bollmann A. ; Funke J. ; Oehlmann J. ; Prasse C. ; Schulte-Oehlmann U. ; Seitz W. ; Ternes T. ; Weber M. ; Wesely H. ; Wagner M.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 47
结束页码: 60
语种: 英语
英文关键词: Activated carbon
; Advanced treatment
; Endocrine disrupting chemicals
; Micropollutants
; Ozonation
; Tertiary treatment
; Transformation products
Scopus关键词: Acidification
; Activated carbon
; Activated carbon treatment
; Chemical analysis
; Effluents
; Endocrine disrupters
; Groundwater
; Multiobjective optimization
; Organic pollutants
; Ozone water treatment
; Ozonization
; Pareto principle
; Phase separation
; Potable water
; Surface waters
; Toxicity
; Water filtration
; Water pollution
; Water quality
; Advanced treatment
; Endocrine disrupting chemicals
; Micropollutants
; Tertiary treatment
; Transformation products
; Extraction
; androgen
; aromatic hydrocarbon
; dioxin
; estrogen
; ground water
; retinoic acid
; sorbent
; surface water
; vitamin D
; activated carbon
; bioassay
; concentration (composition)
; drinking water
; endocrine disruptor
; extraction method
; genotoxicity
; laboratory method
; mutagenicity
; optimization
; ozonation
; sampling
; wastewater
; wastewater treatment
; water
; water quality
; Article
; bioassay
; comparative study
; controlled study
; cytotoxicity
; endocrine function
; genotoxicity
; genotoxicity assay
; human
; in vitro study
; municipal wastewater
; mutagenic activity
; mutagenicity
; priority journal
; solid phase extraction
; suspended particulate matter
; waste water management
; water contamination
; water cycle
; water quality
英文摘要: The assessment of water quality is crucial for safeguarding drinking water resources and ecosystem integrity. To this end, sample preparation and extraction is critically important, especially when investigating emerging contaminants and the toxicity of water samples. As extraction methods are rarely optimised for bioassays but rather adopted from chemical analysis, this may result in a misrepresentation of the actual toxicity. In this study, surface water, groundwater, hospital and municipal wastewater were used to characterise the impacts of common sample preparation techniques (acidification, filtration and solid phase extraction (SPE)) on the outcomes of eleven in vitro bioassays. The latter covered endocrine activity (reporter gene assays for estrogen, androgen, aryl-hydrocarbon, retinoic acid, retinoid X, vitamin D, thyroid receptor), mutagenicity (Ames fluctuation test), genotoxicity (umu test) and cytotoxicity. Water samples extracted using different SPE sorbents (Oasis HLB, Supelco ENVI-Carb+, Telos C18/ENV) at acidic and neutral pH were compared for their performance in recovering biological effects. Acidification, commonly used for stabilisation, significantly altered the endocrine activity and toxicity of most (waste)water samples. Sample filtration did not affect the majority of endpoints but in certain cases affected the (anti-)estrogenic and dioxin-like activities. SPE extracts (10.4 × final concentration), including WWTP effluents, induced significant endocrine effects that were not detected in aqueous samples (0.63 × final concentration), such as estrogenic, (anti-)androgenic and dioxin-like activities. When ranking the SPE methods using multivariate Pareto optimisation an extraction with Telos C18/ENV at pH 7 was most effective in recovering toxicity. At the same time, these extracts were highly cytotoxic masking the endpoint under investigation. Compared to that, extraction at pH 2.5 enriched less cytotoxicity. In summary, our study demonstrates that sample preparation and extraction critically affect the outcome of bioassays when assessing the toxicity of water samples. Depending on the water matrix and the bioassay, these methods need to be optimised to accurately assess water quality. © 2019 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122023
Appears in Collections: 气候变化事实与影响
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作者单位: Department Aquatic Ecotoxicology, Goethe University Frankfurt am Main, Max-von-Laue-Str. 13, Frankfurt, D-60438, Germany; Zweckverband Landeswasserversorgung, Am Spitzigen Berg 1, Langenau, D-89129, Germany; IWW Rheinisch-Westfälisches Institut für Wasser Beratungs- und Entwicklungsgesellschaft mbH, Moritzstraße 26, Muelheim an der Ruhr, D-45476, Germany; Department of Environmental Health and Engineering, Johns Hopkins University, Baltimore, MD 21218, United States; Federal Institute of Hydrology (BfG), Am Mainzer Tor 1, Koblenz, D-56068, Germany; Department of Numerical Analysis and Modelling, Konrad-Zuse-Zentrum für Informationstechnik Berlin (ZIB), Takustraße 7, Berlin, D-14195, Germany; Department of Biology, Norwegian University of Science and Technology, Trondheim, NO-7491, Norway
Recommended Citation:
Abbas A.,Schneider I.,Bollmann A.,et al. What you extract is what you see: Optimising the preparation of water and wastewater samples for in vitro bioassays[J]. Water Research,2019-01-01