globalchange  > 气候变化与战略
DOI: 10.1073/pnas.2011511117
论文题名:
RNA abasic sites in yeast and human cells
作者: Liu Y.; Rodriguez Y.; Ross R.L.; Zhao R.; Watts J.A.; Grunseich C.; Bruzel A.; Li D.; Burdick J.T.; Prasad R.; Crouch R.J.; Limbach P.A.; Wilson S.H.; Cheung V.G.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:34
起始页码: 20689
结束页码: 20695
语种: 英语
英文关键词: Apurinic/apyrimidinic endonuclease 1 ; Methylpurine DNA glycosylase ; R-loop ; RNA ; RNA abasic site
Scopus关键词: deoxyribonuclease I ; DNA glycosyltransferase ; messenger RNA ; ribosome RNA ; deoxyribonuclease I ; DNA ; DNA (apurinic or apyrimidinic site) lyase ; DNA glycosyltransferase ; nucleotide ; RNA ; Article ; base excision repair ; biotinylation ; chemical bond ; controlled study ; DNA RNA hybridization ; electrophilicity ; fungal strain ; human ; human cell ; mass spectrometry ; nonhuman ; priority journal ; protein RNA binding ; RNA processing ; Saccharomyces cerevisiae ; scanning electron microscopy ; yeast cell ; binding site ; chemistry ; DNA damage ; DNA repair ; enzyme specificity ; genetics ; metabolism ; nucleotide sequence ; Saccharomyces cerevisiae ; yeast ; Base Sequence ; Binding Sites ; Deoxyribonuclease I ; DNA ; DNA Damage ; DNA Glycosylases ; DNA Repair ; DNA-(Apurinic or Apyrimidinic Site) Lyase ; Humans ; Nucleotides ; R-Loop Structures ; RNA ; Saccharomyces cerevisiae ; Substrate Specificity ; Yeasts
英文摘要: RNA abasic sites and the mechanisms involved in their regulation are mostly unknown; in contrast, DNA abasic sites are well-studied. We found surprisingly that, in yeast and human cells, RNA abasic sites are prevalent. When a base is lost from RNA, the remaining ribose is found as a closed-ring or an open-ring sugar with a reactive C1′ aldehyde group. Using primary amine-based reagents that react with the aldehyde group, we uncovered evidence for abasic sites in nascent RNA, messenger RNA, and ribosomal RNA from yeast and human cells. Mass spectroscopic analysis confirmed the presence of RNA abasic sites. The RNA abasic sites were found to be coupled to R-loops. We show that human methylpurine DNA glycosylase cleaves N-glycosidic bonds on RNA and that human apurinic/apyrimidinic endonuclease 1 incises RNA abasic sites in RNA–DNA hybrids. Our results reveal that, in yeast and human cells, there are RNA abasic sites, and we identify a glycosylase that generates these sites and an AP endonuclease that processes them. © 2020 National Academy of Sciences. All rights reserved.
Citation statistics:
被引频次[WOS]:22   [查看WOS记录]     [查看WOS中相关记录]
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163409
Appears in Collections:气候变化与战略

Files in This Item:

There are no files associated with this item.


作者单位: Liu, Y., HHMI, University of Michigan, Ann Arbor, MI 48109, United States, Department of Pediatrics, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, United States; Rodriguez, Y., National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, United States; Ross, R.L., Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, United States; Zhao, R., Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, United States; Watts, J.A., Department of Medicine, University of Michigan, Ann Arbor, MI 48109, United States; Grunseich, C., National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892, United States; Bruzel, A., HHMI, University of Michigan, Ann Arbor, MI 48109, United States, Department of Pediatrics, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, United States; Li, D., HHMI, University of Michigan, Ann Arbor, MI 48109, United States, Department of Pediatrics, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, United States; Burdick, J.T., HHMI, University of Michigan, Ann Arbor, MI 48109, United States, Department of Pediatrics, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, United States; Prasad, R., National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, United States; Crouch, R.J., Eunice Kennedy Shriver National Institute of Child Health and Development, NIH, Bethesda, MD 20892, United States; Limbach, P.A., Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, United States; Wilson, S.H., National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, United States; Cheung, V.G., HHMI, University of Michigan, Ann Arbor, MI 48109, United States, Department of Pediatrics, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, United States

Recommended Citation:
Liu Y.,Rodriguez Y.,Ross R.L.,et al. RNA abasic sites in yeast and human cells[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(34)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu Y.]'s Articles
[Rodriguez Y.]'s Articles
[Ross R.L.]'s Articles
百度学术
Similar articles in Baidu Scholar
[Liu Y.]'s Articles
[Rodriguez Y.]'s Articles
[Ross R.L.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu Y.]‘s Articles
[Rodriguez Y.]‘s Articles
[Ross R.L.]‘s Articles
Related Copyright Policies
Null
收藏/分享
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.