globalchange  > 气候变化与战略
DOI: 10.1073/pnas.2003667117
论文题名:
Cofactor-enabled functional expression of fruit fly; honeybee; and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions
作者: Ihara M.; Furutani S.; Shigetou S.; Shimada S.; Niki K.; Komori Y.; Kamiya M.; Koizumi W.; Magara L.; Hikida M.; Noguchi A.; Okuhara D.; Yoshinari Y.; Kondo S.; Tanimoto H.; Niwa R.; Sattelle D.B.; Matsuda K.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:28
起始页码: 16283
结束页码: 16291
语种: 英语
英文关键词: Bumblebee ; Fruit fly ; Honeybee ; Neonicotinoids ; Nicotinic acetylcholine receptors
Scopus关键词: clothianidin ; imidacloprid ; membrane protein ; neonicotinoid ; nicotinic receptor ; thiacloprid ; thioredoxin related transmembrane protein 3 ; unclassified drug ; acetylcholine ; insect protein ; insecticide ; neonicotinoid ; nicotinic agent ; nicotinic receptor ; thioredoxin ; Apis mellifera ; Article ; Bombus terrestris ; controlled study ; Drosophila melanogaster ; nonhuman ; oocyte ; priority journal ; protein expression ; protein function ; Xenopus laevis ; animal ; bee ; dose response ; genetics ; metabolism ; protein subunit ; Acetylcholine ; Animals ; Bees ; Dose-Response Relationship, Drug ; Drosophila melanogaster ; Insect Proteins ; Insecticides ; Neonicotinoids ; Nicotinic Agonists ; Oocytes ; Protein Subunits ; Receptors, Nicotinic ; Thioredoxins ; Xenopus laevis
英文摘要: The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollinators. We show that thioredoxin-related transmembrane protein 3 (TMX3) is essential to enable robust expression in Xenopus laevis oocytes of honeybee (Apis mellifera) and bumblebee (Bombus terrestris) as well as fruit fly (Drosophila melanogaster) nAChR heteromers targeted by neonicotinoids and not hitherto robustly expressed. This has enabled the characterization of picomolar target site actions of neonicotinoids, findings important in understanding their toxicity. © 2020 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/164137
Appears in Collections:气候变化与战略

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作者单位: Ihara, M., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Furutani, S., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Shigetou, S., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Shimada, S., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Niki, K., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Komori, Y., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Kamiya, M., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Koizumi, W., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Magara, L., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Hikida, M., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Noguchi, A., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Okuhara, D., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Yoshinari, Y., Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan; Kondo, S., Genetic Strains Research Center, National Institute of Genetics, Mishima, Shizuoka, 411-8540, Japan; Tanimoto, H., Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, 980-8577, Japan; Niwa, R., Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan; Sattelle, D.B., Centre for Respiratory Biology, University College London Respiratory, University College London, London, WC1E 6JF, United Kingdom; Matsuda, K., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan, Agricultural Technology and Innovation Research Institute, Kindai University, Nara, 631-8505, Japan

Recommended Citation:
Ihara M.,Furutani S.,Shigetou S.,et al. Cofactor-enabled functional expression of fruit fly; honeybee; and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(28)
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