DOI: 10.1016/j.scib.2021.06.014
论文题名: A monocot-specific hydroxycinnamoylputrescine gene cluster contributes to immunity and cell death in rice
作者: Fang H. ; Shen S. ; Wang D. ; Zhang F. ; Zhang C. ; Wang Z. ; Zhou Q. ; Wang R. ; Tao H. ; He F. ; Yang C. ; Peng M. ; Jing X. ; Hao Z. ; Liu X. ; Luo J. ; Wang G.-L. ; Ning Y.
刊名: Science Bulletin
ISSN: 20959273
出版年: 2021
语种: 英语
中文关键词: bZIP transcription factor
; Cell death
; HP gene cluster
; Magnaporthe oryzae
; Oryza sativa
; Plant immunity
; Secondary metabolites
英文关键词: Amino acids
; Biochemistry
; Biosynthesis
; Cell death
; Encoding (symbols)
; Metabolites
; Signal encoding
; Transcription factors
; Biosynthetic gene cluster
; bZIP transcription factors
; Encodings
; Gene clusters
; Hydroxycinnamoylputrescine gene cluster
; Magnaporthe oryzae
; Monocots
; Oryza sativa
; Plant immunities
; Secondary metabolites
; Transcription
英文摘要: Phenolamides (PAs), a diverse group of specialized metabolites, including hydroxycinnamoylputrescine (HP), hydroxycinnamoylagmatine, and hydroxycinnamoyltryptamine, are important in plant resistance to biotic stress. However, the genes involved in the biosynthesis and modulation of PAs have not been fully elucidated. This study identified an HP biosynthetic gene cluster in rice (Oryza sativa) comprising one gene (OsODC) encoding a decarboxylase and two tandem-duplicated genes (OsPHT3 and OsPHT4) encoding putrescine hydroxycinnamoyl acyltransferases coexpressed in different tissues. OsODC catalyzes the conversion of ornithine to putrescine, which is used in HP biosynthesis involving OsPHT3 and OsPHT4. OsPHT3 or OsPHT4 overexpression causes HP accumulation and cell death and putrescine hydroxycinnamoyl acyltransferases (PHT) activity-dependent resistance against the fungal pathogen Magnaporthe oryzae. OsODC overexpression plants also confer enhanced resistance to M. oryzae. Notably, the basic leucine zipper transcription factor APIP5, a negative regulator of cell death, directly binds to the OsPHT4 promoter, repressing its transcription. Moreover, APIP5 suppression induces OsPHT4 expression and HP accumulation. Comparative genomic analysis revealed that the HP biosynthetic gene cluster is conserved in monocots. These results characterized a previously unidentified monocot-specific gene cluster that is involved in HP biosynthesis and contributes to defense and cell death in rice. © 2021 Science China Press Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/170239
Appears in Collections: 气候变化与战略
There are no files associated with this item.
作者单位: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; College of Agronomy, Hunan Agricultural University, Changsha, 410128, China; National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China; Department of Plant Pathology, The Ohio State University, Columbus, OH 43210, United States; College of Tropical Crops, Hainan University, Haikou, 572208, China
Recommended Citation:
Fang H.,Shen S.,Wang D.,et al. A monocot-specific hydroxycinnamoylputrescine gene cluster contributes to immunity and cell death in rice[J]. Science Bulletin,2021-01-01