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DOI:	10.1371/journal.pone.0142079
论文题名:	Widespread Environmental Contamination with Mycobacterium tuberculosis Complex Revealed by a Molecular Detection Protocol
作者:	Nuno Santos; Catarina Santos; Teresa Valente; Christian Gortázar; Virgílio Almeida; Margarida Correia-Neves
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2015
发表日期:	2015-11-11
卷:	10, 期:11
语种:	英语
英文关键词:	Polymerase chain reaction ; Sediment ; DNA extraction ; Mycobacterium bovis ; Surface water ; Deer ; Mycobacterium tuberculosis ; Wildlife
英文摘要:	Environmental contamination with Mycobacterium tuberculosis complex (MTC) has been considered crucial for bovine tuberculosis persistence in multi-host-pathogen systems. However, MTC contamination has been difficult to detect due to methodological issues. In an attempt to overcome this limitation we developed an improved protocol for the detection of MTC DNA. MTC DNA concentration was estimated by the Most Probable Number (MPN) method. Making use of this protocol we showed that MTC contamination is widespread in different types of environmental samples from the Iberian Peninsula, which supports indirect transmission as a contributing mechanism for the maintenance of bovine tuberculosis in this multi-host-pathogen system. The proportion of MTC DNA positive samples was higher in the bovine tuberculosis-infected than in presumed negative area (0.32 and 0.18, respectively). Detection varied with the type of environmental sample and was more frequent in sediment from dams and less frequent in water also from dams (0.22 and 0.05, respectively). The proportion of MTC-positive samples was significantly higher in spring (p<0.001), but MTC DNA concentration per sample was higher in autumn and lower in summer. The average MTC DNA concentration in positive samples was 0.82 MPN/g (CI95 0.70–0.98 MPN/g). We were further able to amplify a DNA sequence specific of Mycobacterium bovis/caprae in 4 environmental samples from the bTB-infected area.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0142079&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/20795
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0142079.PDF（746KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View 联系获取全文

作者单位:

Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal;ICVS/3B’s, PT Government Associate Laboratory, Braga/Guimarães, Portugal;Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal;ICVS/3B’s, PT Government Associate Laboratory, Braga/Guimarães, Portugal;Earth Sciences Institute (ICT), Pole of the University of Minho, Earth Sciences Department, University of Minho, Braga, Portugal;SaBio (Health and Biotechnology), IREC, National Wildlife Research Institute (CSIC-UCLM-JCCM), Ciudad Real, Spain;Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa (FMV-ULisboa), Lisboa, Portugal;Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal;ICVS/3B’s, PT Government Associate Laboratory, Braga/Guimarães, Portugal

Recommended Citation:

Nuno Santos,Catarina Santos,Teresa Valente,et al. Widespread Environmental Contamination with Mycobacterium tuberculosis Complex Revealed by a Molecular Detection Protocol[J]. PLOS ONE,2015-01-01,10(11)

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