| 项目编号: | 1704085
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| 项目名称: | CREWS: Chemical Resonance Excitation Wavelength Selection for Label-Free DNA Analysis |
| 作者: | Yeshaiahu Fainman
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| 承担单位: | University of California-San Diego
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| 批准年: | 2017
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| 开始日期: | 2017-07-01
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| 结束日期: | 2020-06-30
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| 资助金额: | 400000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | dna strand
; genomic mutation
; dna sequencing
; multiple excitation wavelength
; specific gene
; antimicrobial resistance
; chemical resonance excitation wavelength selection
; multiwavelength surface-enhanced raman spectroscopy
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| 英文摘要: | This project will develop a multiwavelength surface-enhanced Raman spectroscopy (SERS) method for determining genomic mutations within specific genes related to antimicrobial resistance. The PI has published convincing preliminary results on extracting relevant information related to the composition of DNA strands from the measured SERS spectra. The ability to rapidly determining the genomic mutations within specific genes related to antimicrobial resistance would have a significant societal impact.mThe project will provide scientific training for students at graduate and undergraduate levels across several multidisciplinary fields, exposing them to advanced photonics, nanoscale fabrication, surface chemistry, and medical diagnostics.
The goal of this project is to develop the chemical resonance excitation wavelength selection (CREWS) method for determining genomic mutations within specific genes related to antimicrobial resistance (AMR). The proposed platform employs multiple excitation wavelengths to maximize the amount of data acquired from DNA using Raman spectroscopy, and uses advanced signal processing techniques to identify mutations within genes responsible for causing AMR. This technique provides an alternative approach to genomic mutation detection without the need for DNA sequencing, in which quick and efficient label-free detection of single point mutations in DNA strands can be performed. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/89885
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Yeshaiahu Fainman. CREWS: Chemical Resonance Excitation Wavelength Selection for Label-Free DNA Analysis. 2017-01-01.
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