| 项目编号: | 1701918
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| 项目名称: | DISSERTATION RESEARCH: The Evolution of Plant Drought Tolerance and Gene Function Across Historic Frequency Gradients |
| 作者: | John McKay
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| 承担单位: | Colorado State University
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| 批准年: | 2017
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| 开始日期: | 2017-06-01
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| 结束日期: | 2018-05-31
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| 资助金额: | 19760
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Biological Sciences - Environmental Biology
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| 英文关键词: | gene
; historic drought frequency
; drought
; reverse genetics
; drought tolerance trait
; different drought frequency
; plant arabidopsis thaliana
; basic evolutionary process
; important drought tolerance trait
; gene function
; function allele
; plant evolution
; t-dna gene knockout
; plant population
; drought tolerant crop
; drought tolerance
; drought adaptation
; genetic difference
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| 英文摘要: | This research will investigate the function of genes possibly involved in drought adaptation in wild populations of the plant Arabidopsis thaliana. Droughts are one of nature?s most devastating forces, affecting millions of people each year. Yet as agents of strong selection on plant populations, they are also valuable for understanding basic evolutionary processes, such as discovering the genes involved in adaptation. Furthermore, studying plant evolution in the context of drought may produce the critical knowledge needed to develop drought tolerant crops. Using a global map of historic drought frequency based on remote sensing data, populations were identified that experience very different drought frequencies. From data on genetic differences among those populations, 20 genes were identified that will be studied for their molecular and phenotypic effects. This work is expected to yield key insight into the evolution of gene function, and to confirm genes underlying drought tolerance traits. The results of studying these genes may be of great value to breeding programs working to improve drought tolerance in crop species. In addition, the project will involve mentoring both undergraduate and high school students, including individuals from groups that are underrepresented in the sciences.
Historic drought frequency significantly predicts important drought tolerance traits, flowering time and water use efficiency, among natural accessions of Arabidopsis thaliana. The project will investigate the effects of loss of function alleles on 20 genes involved in flowering time or water use efficiency. A reverse genetics approach based on T-DNA gene knockouts will be used to artificially induce mutations in these genes in a common genomic background. The project will quantify the effect sizes of these hypothesized genes on flowering time and water use efficiency. It will also characterize effects on whole genome expression profiles and abiotic signaling cascades using RNAseq. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/90129
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
John McKay. DISSERTATION RESEARCH: The Evolution of Plant Drought Tolerance and Gene Function Across Historic Frequency Gradients. 2017-01-01.
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